Programmbereich Asthma und Allergie

Allergobiochemie

Mission    Projekte    Drittmittel    Methoden    Publikationen    Mitarbeiter/innen

Techniques

The group of Allergobiochemistry possess a strong expertise in the isolation, purification protocols of different environmental samples, along with the chemical and structural characterization of the obtained compounds. Moreover, we have experience in immunological test of technically challenging lipid molecules.

  • Various extraction/purification protocols aiming for isolation and purification of components of microbial and environmental origin (dust, house dust mites, pollen).
  • Various chemical analyses, aiming for the compositional analyses of isolated carbohydrates, glycolipids, lipids
  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
  • Western blot
  • High Performance-Thin Layer Chromatography (TLC, HPTLC)
  • Ultracentrifugation
  • Gel permeation chromatography
  • Hydrophobic-interaction chromatography
  • Affinity chromatography
  • Anion exchange chromatography
  • Gas chromatography (GC)
  • Gas chromatography-Mass spectrometry (GC-MS)
  • High-performance liquid chromatography (HPLC)
  • High-performance anion-exchange chromatography (HPAEC)
  • NMR spectroscopy
  • Mass spectrometry (in collaboration with the division of Bioanalytical Chemistry)
  • Flow cytometry
  • Cell culture
  • ELISA
  • RT-PCR
  • Human and mouse immune cell isolation