Programmbereich Asthma und Allergie

Experimentelle Pneumologie

Mission    Projekte    Drittmittel    Methoden    Publikationen    Mitarbeiter/innen

Techniques

Analysis of Lung function

  • invasive lung function analysis in mice


Analysis of Lung Structure

  • design-based stereology
  • fluorescence and immuno histochemistry
  • glycol methacrylate embedding


Analysis of Lung Cell and Molecular Biology

  • airway microdissection
  • airway explant culture
  • air-liquid-interface culture of primary airway epithelial cells
  • isolation and differentiation of murine Th17 cells
  • enzyme-linked immune sorbent assay (ELISA)
  • cytometric bead array s(CBA)
  • magnetic cell sorting
  • flow cytometry
  • western blotting
  • RNA-isolation, cDNA synthesis, quantitative real-time RT-PCR
  • isolation and stimulation of primary murine mast cells
  • analysis of mast cell activation and degranulation by flow cytometry


Protein Biochemistry

  • protein purification and chromatographic techniques
  • protein crystallography
  • recombinant protein expression (E. coli, insect cells, HEK cells)


Culture of Human Cells

  • isolation and co-culture of human peripheral blood immune cells (special expertise: basophils).
  • production and purification of monoclonal antibodies
  • air liquid-interface culture of primary airway epithelial cells