Priority Research Area Asthma and Allergy

Invertebrate Models

Mission    Projects   Funding   Techniques   Publications    Staff

Techniques

Molecular biology

  • RNA isolation, cDNA synthesis, PCR, quantitative Real-time PCR (Lightcycler 480)
  • Cloning techniques
  • DNA microarray
  • Enhancer-trap technique


Cell- and immunobiology

  • Fluorescence- and immunocytology/ -histology
  • Collection of hemocytes (phagocytes) and sampling of hemolymph plasma
  • Microinjection of bacteria


Drosophila genetics

  • Generating of transgenic strains using different expression systems: Bipartite Gal4/ UAS system, TARGET system, GeneSwitch Gal4-System
  • Establishing of transgenic Drosophila strains