Cultivation of hybridoma, production and purification of monoclonal antibodies
Cultivation, preparation and identification of BSL2 and BSL3 pathogens (e.g. Mycobacterium tuberculosis or Trypanosoma cruzi)
Infection of experimental mice (subcutaneous, intravenous, intraperitoneal, aerosol infection with mycobacteria)
In vitro differentiation, infection and analysis of bone marrow derived macrophages and dendritic cells
In vitro differentiation of TH1, TH2 and TH17 cells
Isolation of organs from experimental mice, preparation of single cell suspension and cell sorting
Isolation of cells and granulomas from perfused lungs and livers of infected animals
Magnetic cell sorting
Flowcytometry and immunofluorescence
Histological and immunohistochemical techniques
Confocal microscopy
Quantitative RT-PCR, real time PCR
Detection of antibodies, cytokines and chemokines intermediates in tissue culture supernatants, tissue homogenates and serum/plasma
Cytometric bead arrays
Determination of reactive nitrogen intermediates and quantification of arginase activity
Cytokine bioassays
Functional T cell assays (proliferation, antigen-specific recall and cytokine-secretion assays, ELISPOT, CTL assays, preparation of Mtb-specific class I and class II tetramers and staining of specific CD4+ and CD8+ T cells)
Projects