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Fluorescence Cytometry

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The Fluorescence Cytometry is a core facility providing up-to-date equipment in the field of microscopy and flow cytometry for both Priority Research Areas of the center. In addition to care and maintenance of the equipment, the practical and theoretical training of employees is a main focus. Other services include counseling and assistance in experimental design and analysis. For complex methodical approaches cooperations with the respective research groups are carried out.

For microscopical studies there is currently a confocal laser scanning microscope Leica TCS SP5 with five lasers available. The device permits free selection of wavelength ranges for detection and is equipped with three photomultipliers and two very sensitive hybrid detectors. In addition, for live cell analysis, an Olympus IX-81 system can be used.

Chipcytometry enables the analysis of a virtually unlimited number of biomarkers on single-tissue or cell suspension samples. Additionally samples can be preservd and stored/shipped for controlled centralized analysis without significant biomarker degradation.

Flow cytometric analysis can be carried out utilizing a BD FACSCalibur or a MACSQuant10 and two well-equipped BD LSRII cytometers. With the later device, using four excitation lasers, up to 18 fluorochromes can be measured simultaneously. In addition, a cell sorting service is offered employing a BD FACSAriaIIIu. This cell sorter provides four excitation lasers and 13 fluorescence channels (by the use of a bioBUBBLE, biosafety level 2 sorts are possible).

Scientists at the Research Center Borstel have also access to flow cytometric equipment within the biosafety level 3 laboratory. Analyzes can be carried out on a BD FACSCantoII (3 lasers, 8 fluorescence channels), or a MACSQuant10 (3 lasers, 8 fluorescence channels). Within the BSL3 also a BioRad S3 cell sorter will is available, which is equipped with two lasers and four fluorescence channels.

The Fluorescence Cytometry unit is jointly managed by Dr. Jochen Behrends and Dr. Thomas Scholzen.

Olympus IX-81 (PA22, R35)