Service

Fluorescence Cytometry

Mission   Projects   Publications    Staff

Projects

The Fluorescence Cytometry is a core facility providing up-to-date equipment in the field of microscopy and flow cytometry for both Priority Research Areas of the center. In addition to care and maintenance of the equipment, the practical and theoretical training of employees is a main focus. Other services include counseling and assistance in experimental design and analysis. For complex methodical approaches cooperations with the respective research groups are carried out.

For microscopical studies there is currently a confocal laser scanning microscope Leica TCS SP5 with five lasers available. The device permits free selection of wavelength ranges for detection and is equipped with three photomultipliers and two very sensitive hybrid detectors. In addition, for live cell analysis, an Olympus IX-81 system can be used.

Chipcytometry enables the iterative analysis of biomarkers on single-tissue or cell suspension samples. Additionally, samples can be preserved and stored/shipped for controlled centralized analysis without significant biomarker degradation.

Flow cytometric analysis can be carried out on instruments with 12-21 fluorescence parameters including a FACSymphony A1, which is specially equipped with a small particle detector (SPD), allowing the detection of small particles down to at least 60nm.

In addition, a cell sorting service is offered employing a BD FACSAriaIIIu. This cell sorter provides four excitation lasers and 13 fluorescence channels (by the use of a bioBUBBLE, biosafety level 2 sorts are possible).

In autumn 2023, the facility is able to provide the users with the FACSDiscover S8 with cell view image technology; 6-way sortings are possible, using 78 spectral parameters in addition with image detectors (including three fluorescence channels).

Scientists at the Research Center Borstel have also access to flow cytometric equipment within the biosafety level 3 laboratory. Analyzes can be carried out on a MACSQuant10 (3 lasers, 8 fluorescence channels). Within the BSL3 also a BioRad S3 cell sorter is available, which is equipped with two lasers and four fluorescence channels.

The Fluorescence Cytometry unit is jointly managed by Dr. Jochen Behrends and Dr. Thomas Scholzen.

• Category Title

Olympus IX-81

Leica TCS Sp5

ZellScanner ONE (Canopy Biosciences)

MACSQuant 10 – BSL3 (Miltenyi Biotech; 3 laser, 8 colors)

MACSQuant 16 (Miltenyi Biotech; 3 laser, 14 colors)

FACSLyric (BD Biosciences; 3 laser, 12 colors)

S3 Cell Sorter – BSL3 (Bio-Rad; 2 laser, 4 colors)

FACSymphony A1 (BD Biosciences; 4 laser, 16 colors; small particle detector)

FACSymphony A3 (BD Biosciences; 4 laser, 21 colors)

FACSAria IIIu – BSL2-bioBUBBLE (BD Biosciences, 4 laser, 13 colors)

FACSDiscover S8 Cell Sorter with CellView Image Technology (BD Biosciences; 5 laser, 78 spectral detectors; 3-color fluorescence imaging; 6-way sorting)