Priority Research Area Asthma and Allergy

Early Life Origins of CLD

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Techniques

Standard molecular and cellular methods are not detailed here (qPCR, Western blotting, flow cytometry, cell culture, histology  etc)

Special techniques

  • Animal models
    • A: Exposure to cigarette smoke, mimicking active or passive smoking
    • B: Asthma models acute/chronic using HDM/ovalbumin
    • C: Transgenerational animal models: in utero smoking
      • A and B include online monitoring of total particulate matter and detailed phenotypic  and molecular characterization of mothers and offspring (cotinine levels, CO-Hb, invasive lung function testing, molecular networks and immunology, propensity to asthma development in offfspring )
  • MicroRNA profiling in sera and tissues
  • Air liquid interface culture of human bronchial cells with induction of asthma-like phenotype
  • Isolation and functional characterization of Exosomes


For details of experimental protocols contact
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