Histology
Main Emphasis
Within the Core Unit, we carry out histological work on animal models generated in FZB projects. In addition to consulting on pre-analysis, we process tissue for embedding in paraffin, preparation of sections, histological staining, immunohistochemistry and archiving of block material. Frozen sections are also part of our tasks. In the field of archieving, we work closely with BMB Nord. We consult the various research groups on the planning and execution of histological analyzes as well as further investigations such as transcriptomics, multispectral IHC, nanostring and spatial transcriptomics.
Research
As part of the German Center for Lung Research (DZL) at the North site (ARCN), we are involved in third-party funded projects in the disease areas lung cancer, COPD, asthma/allergy and biobanking. Our particular research focus is the TGF-beta signaling pathway in NSCLC. Moreover, embryological processes and the tumor microenvironment are gaining increasing relevance. After years of basic research, we are now intensively engaged in translating the results obtained in this field into clinical practice. In the area of infection, we are particularly interested in the multispectral immunohistochemical analysis of pathophysiological events in tuberculosis granulomas and transcriptome studies within various model systems and cohorts, with the aim of developing host directed therapies.
- DZL LC central project Tumor microenvironment in Lung Cancer
Cooperation with R. Savai (MPI Bad Nauheim), Univ. Heidelberg (M. Thomas, H. Winther, P. Christopoulos), DKFZ (U. Klingmüller), Lungenclinic Grosshansdorf (M. Reck, K.F Rabe, H. Watz), Univ. Lübeck (D. Drömann). Univ. München (A. Tufmann). - DZL-DZIF overarching project: Multispectral Immunohistochemistry, Transcriptomics and Epigenetics in Tuberculosis
Cooperation with A. Blumenthal (Univ. Queensland, Australia), T. Dallenga, U. Schaible, N. Shubladze (Univ. Tblisi, Georgia) C. Lange, Ch. Hölscher, S. Niemann and N. Reiling. - DZL LC basic science flagship project: TGF beta signaling in NSCLC
Cooperation with MPI Bad Nauheim (R. Savai, Pirfenidone), CPC-München (G. Stathopoulos, SMAD-Linker) und DKFZ (U. Klingmüller) and LungenClinic Grosshansdorf (M.Reck, K.F. Rabe), Univ. Lübeck (D. Drömann). - DZL LC translational flagship project: Epigenetics of NSCLC
Cooperation with Univ. Ulm (O. Ammerpohl), Lungenclinic Grosshansdorf (M. Reck, K.F. Rabe) - DZL LC COPD and AA
Cooperation with LungenClinic (H. Watz, K.F. Rabe), Univ. Ulm (O. Ammerpohl), D. Schwudke, S. Krauss-Etschmann, U. Jappe, S. Meiners, H. Heine, K. Stein, M. Wegmann, F. Petersen, A. Frey, H. Fehrenbach and K. Brandenburg. - BMBF Indicate FH
Histology, Transcriptomics and Methylomes in Wheat Allergy and Hypersensitivity. Cooperation with U. Jappe, Th. Kufer (Univ. Stuttgart), S. Bischoff (Univ. Stuttgart), Christian Sina (Univ. Lübeck).
BMBF
- German Center for Lung Research (DZL), Airway Research Center North (ARCN),
Disease Area LC (Lung Cancer). Project duration: 1.1. 2021 – 12/31/2023. - German Center for Lung Research (DZL), Airway Research Center North (ARCN),
Disease Area COPD (COPD). Project duration: 1.1. 2021 – 12/31/2023. - German Center for Lung Research (DZL), Airway Research Center North (ARCN),
Platform Biobanking. Project duration: 1.1. 2021 – 12/31/2023. - Indicate FH. Project duration: 2022-2025.
We work primarily on histological material. Its processing (FFPE, freeze, HOPE) is our central methodical basis. In addition to conventional histological staining techniques, we frequently and regularly work with immunohistochemical staining methods.
- Multispectral Immunhistochemistry and Spatial Transcriptomics
We are delighted to have a multispectral immunohistochemical platform (Akoya Vectra-Polaris) available through funding from the DZL, which, in addition to our other technologies (transcriptome, nanostring, cell culture), decisively expands the methodological portfolio. In addition, we managed to acquire a platform for spatial transcriptomics (10x) together with the research group early childhood asthma.
We are very delighted to have a multispectral immunohistochemical platform (Akoya Vectra-Polaris) through a DZL funding, which decisively expands the methodological portfolio alongside our other technologies (transcriptome, nanostring, cell culture). In addition, we have succeeded in acquiring a platform for Spatial Transcriptomics (10x) together with the reseach group early childhood asthma. - Transcriptome analyses
In addition to transcriptomics on our DZL-financed Agilent platform (incl. Bioanalyzer and Nanodrop), we offer investigations on the Nanostring N-Counter, providing a prep station. We also apply a light cycler. - Cell culture
We use different cell systems to enable functional studies. In addition to cell lines, this also includes human primary cell cultures (in cooperation with the Grosshansdorf Lung Clinic). Appropriate read out systems are western blot, in cell western and fluorescence microscopy.
2024
Höhne, K, Wagenknecht, A, Maier, C, Engelhard, P, Goldmann, T, Schließmann, SJ, Plönes, T, Trepel, M, Eibel, H & Zissel, G 2024, 'Pro-Fibrotic Effects of CCL18 on Human Lung Fibroblasts Are Mediated via CCR6', Cells, Jg. 13, Nr. 3, 238. https://doi.org/10.3390/cells13030238
Kuempers, C, Schnepf, K, Marwitz, S, Watermann, C, Scheel, A, Fischer, RN, Ammerpohl, O, Perner, S, Drömann, D & Goldmann, T 2024, 'Upregulation and epigenetic modification of the creatine transporter SLC6A8 in non-small cell lung cancer', Histology and histopathology, S. 18731. https://doi.org/10.14670/HH-18-731
Richtmann, S, Marwitz, S, Muley, T, Koistinen, H, Christopoulos, P, Thomas, M, Kazdal, D, Allgäuer, M, Winter, H & Goldmann, T et al. 2024, 'The pregnancy-associated protein glycodelin as a potential sex-specific target for resistance to immunotherapy in non-small cell lung cancer', Translational Research. https://doi.org/10.1016/j.trsl.2024.02.007
2023
Chen, J, Wang, X, Schmalen, A, Haines, S, Wolff, M, Ma, H, Zhang, H, Stoleriu, MG, Nowak, J, Nakayama, M, Bueno, M, Brands, J, Mora, AL, Lee, JS, Krauss-Etschmann, S, Dmitrieva, A, Frankenberger, M, Hofer, TP, Noessner, E, Moosmann, A, Behr, J, Milger, K, Deeg, CA, Staab-Weijnitz, CA, Hauck, SM, Adler, H, Goldmann, T, Gaede, KI, Behrends, J, Kammerl, IE & Meiners, S 2023, 'Antiviral CD8+ T cell immune responses are impaired by cigarette smoke and in COPD', The European respiratory journal, Jg. 62, Nr. 2, 2201374. https://doi.org/10.1183/13993003.01374-2022
GEN-COVID Multicenter Study Group 2023, 'CYP19A1 mediates severe SARS-CoV-2 disease outcome in males', Cell reports. Medicine, S. 101152. https://doi.org/10.1016/j.xcrm.2023.101152
2022
Shan, L, Zhang, L, Zhu, X, Wang, Z, Fang, S, Lin, J, Wang, J, Li, N, Liu, H, Zhang, X, Feng, Y, Liu, J, Pan, J, Ye, G, Yu, X, Tufman, A, Katalinic, A, Goldmann, T, Petersen, F, Jiang, J, Geng, G & Yu, X 2022, 'Chinese never smokers with adenocarcinoma of the lung are younger and have fewer lymph node metastases than smokers', RESPIRATORY RESEARCH, Jg. 23, Nr. 1, S. 293. https://doi.org/10.1186/s12931-022-02199-z
Kasper, B, Yue, X, Goldmann, T, Gülsen, A, Kugler, C, Yu, X & Petersen, F 2022, 'Air exposure and cell differentiation are essential for investigation of SARS-CoV-2 entry genes in human primary airway epithelial cells in vitro', Frontiers in Medicine, Jg. 9, S. 897695. https://doi.org/10.3389/fmed.2022.897695
DZIF-TB cohort study group 2022, 'Gene expression signatures identify biologically and clinically distinct tuberculosis endotypes', The European respiratory journal, Jg. 60, Nr. 3. https://doi.org/10.1183/13993003.02263-2021
Kupsch, S, Eggers, LF, Spengler, D, Gisch, N, Goldmann, T, Fehrenbach, H, Stichtenoth, G, Krause, MF, Schwudke, D & Schromm, AB 2022, 'Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures', European journal of pharmaceutical sciences, Jg. 175, S. 106216. https://doi.org/10.1016/j.ejps.2022.106216
Budczies, J, Kirchner, M, Kluck, K, Kazdal, D, Glade, J, Allgäuer, M, Kriegsmann, M, Heußel, C-P, Herth, FJ, Winter, H, Meister, M, Muley, T, Goldmann, T, Fröhling, S, Wermke, M, Waller, CF, Tufman, A, Reck, M, Peters, S, Schirmacher, P, Thomas, M, Christopoulos, P & Stenzinger, A 2022, 'Deciphering the immunosuppressive tumor microenvironment in ALK- and EGFR-positive lung adenocarcinoma', CANCER IMMUNOLOGY IMMUNOTHERAPY, Jg. 71, Nr. 2, S. 251-265. https://doi.org/10.1007/s00262-021-02981-w
Uchino, J, Goldmann, T & Kimura, H 2022, 'Editorial: Treatment for Non-Small Cell Lung Cancer in Distinct Patient Populations', Frontiers in Oncology, S. 838570. https://doi.org/10.3389/fonc.2022.838570
Head
Scientific staff
Technical staff
Last Update: 09.01.2024