Priority Research Area Asthma and Allergy

Experimental Pneumology

Mission   Projects   Funding   Techniques   Publications    Staff

Techniques

In-vitro Culture of Primary Cells and Tissues

• air-liquid-interface culture of primary human airway epithelial cells
• microdissection and explant culture of murine proximal and distal airways
• isolation and stimulation of primary murine mast cells
• isolation and co-culture of human peripheral blood immune cells (special expertise: basophils).
• production and purification of monoclonal antibodies

Analysis of Lung Structure

• design-based stereology
• fluorescence and immuno histochemistry

Analysis of Lung Cell and Molecular Biology

• enzyme-linked immune sorbent assay (ELISA)
• cytometric bead array s(CBA)
• magnetic cell sorting
• flow cytometry
• Western blotting
• RNA-isolation, cDNA synthesis, quantitative real-time RT-PCR
• analysis of mast cell activation and degranulation by flow cytometry

Protein Biochemistry

• protein purification and chromatographic techniques
• recombinant protein expression (E. coli, insect cells, HEK cells)