Priority Research Area Asthma and Allergy
Experimental Pneumology
Mission
Projects
Funding
Techniques
Publications
Staff
Techniques
In-vitro Culture of Primary Cells and Tissues
- air-liquid-interface culture of primary human airway epithelial cells
- microdissection and explant culture of murine proximal and distal airways
- isolation and stimulation of primary murine mast cells
- isolation and co-culture of human peripheral blood immune cells (special expertise: basophils).
- production and purification of monoclonal antibodies
Analysis of Lung Structure
- design-based stereology
- fluorescence and immuno histochemistry
Analysis of Lung Cell and Molecular Biology
- enzyme-linked immune sorbent assay (ELISA)
- cytometric bead array s(CBA)
- magnetic cell sorting
- flow cytometry
- Western blotting
- RNA-isolation, cDNA synthesis, quantitative real-time RT-PCR
- analysis of mast cell activation and degranulation by flow cytometry
Protein Biochemistry
- protein purification and chromatographic techniques
- recombinant protein expression (E. coli, insect cells, HEK cells)