Priority Research Area Chronic Lung Diseases

Mucosal Immunology and Diagnostic

Mission   Projects   Funding   Techniques   Publications    Staff

Techniques

Immunological methods:

• Experimental immunizations
• Extraction of immunoglobulins from secretions
• ELISA
• Epitope mapping
• Immunohistochemistry
• Production of monoclonal antibodies
  
  

Cell biological & histological methods:

• Cell culture
• In vivo and ex vivo transport studies at mucosal tissues
• Light and electron microscopy
  
  

Biochemical & chemical methods:

• Synthesis of crosslinkers and tags
• Generation of protein and peptide conjugates
• Solid phase synthesis of peptide libraries
• Immobilization of proteins and peptides on solid surfaces
• Screening of synthetic peptide libraries
• Preparation of mucosal secretions and secreted proteins
• Proteolysis assays
  
  

Bioinformatic methods:

• Evolutionary optimization of peptide ligands
  
  

Molecular biological methods:

• Molecular cloning in E. coli
• Expression and purification of recombinant proteins
• Screening of microbial peptide libraries