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Focus on lungs
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Chronic Lung Diseases
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Infections
Priority Research Area Chronic Lung Diseases
Mucosal Immunology and Diagnostic
Techniques
Immunological methods:
- Experimental immunizations
- Extraction of immunoglobulins from secretions
- ELISA
- Epitope mapping
- Immunohistochemistry
- Production of monoclonal antibodies
Cell biological & histological methods:
- Cell culture
- In vivo and ex vivo transport studies at mucosal tissues
- Light and electron microscopy
Biochemical & chemical methods:
- Synthesis of crosslinkers and tags
- Generation of protein and peptide conjugates
- Solid phase synthesis of peptide libraries
- Immobilization of proteins and peptides on solid surfaces
- Screening of synthetic peptide libraries
- Preparation of mucosal secretions and secreted proteins
- Proteolysis assays
Bioinformatic methods:
- Evolutionary optimization of peptide ligands
Molecular biological methods:
- Molecular cloning in E. coli
- Expression and purification of recombinant proteins
- Screening of microbial peptide libraries