Priority Research Area Chronic Lung Diseases
Pulmonary Immune Diseases
Mission
Projects
Funding
Techniques
Publications
Staff
Techniques
- Animal models for
Systemic sclerosis
Autoimmune bullous dermatoses
Allergic asthma - Lung function analyses
- Ex vivo model for autoimmune bullous dermatoses
- Protein biochemical methods
- Cultivation of hybridomas, production and purification of monoclonal antibodies
- Primary cell culture from human and animal tissues
- Functional analyses of neutrophil granulocytes
- In vitro differentiation and analysis of bone marrow-derived neutrophils, macrophages and dendritic cells
- Isolation of organs from experimental mice, preparation of single cell suspensions
- Magnetic cell sortingFlow cytometry and immunofluorescence
- In-Cell Western Assay
- Histological and immunohistochemical techniques
- Quantitative RT-PCR, real time PCR
- Detection of antibodies, cytokines and chemokines in tissue homogenates, supernatants and serum/plasma
- Cytometric bead arrays
- Determination of reactive oxygen metabolites (ROS)
- Signal transduction