Priority Research Area Asthma and Allergy

Pulmonary Immune Diseases

Mission   Projects  Funding   Techniques   Publications    Staff

Techniques

  • Animal models for
    Systemic sclerosis
    Autoimmune bullous dermatoses
    Allergic asthma
  • Lung function analyses
  • Ex vivo model for autoimmune bullous dermatoses
  • Protein biochemical methods
  • Cultivation of hybridomas, production and purification of monoclonal antibodies
  • Primary cell culture from human and animal tissues
  • Functional analyses of neutrophil granulocytes
  • In vitro differentiation and analysis of bone marrow-derived neutrophils, macrophages and dendritic cells
  • Isolation of organs from experimental mice, preparation of single cell suspensions
  • Magnetic cell sortingFlow cytometry and immunofluorescence
  • In-Cell Western Assay
  • Histological and immunohistochemical techniques
  • Quantitative RT-PCR, real time PCR
  • Detection of antibodies, cytokines and chemokines in tissue homogenates, supernatants and serum/plasma
  • Cytometric bead arrays
  • Determination of reactive oxygen metabolites (ROS)
  • Signal transduction