Priority Research Area Infections

Cellular Microbiology

Mission   Projects   Funding   Techniques   Publications    Staff

Techniques

Infection biology

Infection biology

• Studies under BSL3 safety conditions
• Aerosol infection models for tuberculosis (BSL3)
• Vaccination and drug treatment protocols
• Lung microbiota analysis (16S RNA sequencing), FISH
• In vivo imaging (BSL3)
• In vitro infection models of mouse and human primary cells (BSL3)

    
Infection Immunology

• FACS analysis and sorting (BSL2 and 3)
• Histology, immunohistology
• Cytokine / chemokine assays
• T cell assays
• Real-time PCR
• Small lipid mediator analysis

    
Cellular microbiology

• Intracellular trafficking studies, confocal microscopy
• Life cell imaging (BSL2 and 3)
• Primary cell cultures (human, mouse; macrophages, neutrophils, dendritic cells, lymphocytes, lung epithelial cells)
• Biofilmassays
• Subcellular fractionation, phagosome isolation
• Cell death assays
• siRNA-based knock down

    
Biochemistry

• SDS-PAGE, western blot
• Liposome technology
• Glycolipid isolation, labeling
• Thin layer chromatography