Priority Research Area Infections

Cellular Microbiology

Mission   Projects   Funding   Techniques   Publications    Staff

Techniques


Infection biology

Infection biology

  • Studies under BSL3 safety conditions
  • Aerosol infection models for tuberculosis (BSL3)
  • Vaccination and drug treatment protocols
  • Lung microbiota analysis (16S RNA sequencing), FISH
  • In vivo imaging (BSL3)
  • In vitro infection models of mouse and human primary cells (BSL3)

    
Infection Immunology

  • FACS analysis and sorting (BSL2 and 3)
  • Histology, immunohistology
  • Cytokine / chemokine assays
  • T cell assays
  • Real-time PCR
  • Small lipid mediator analysis

    
Cellular microbiology

  • Intracellular trafficking studies, confocal microscopy
  • Life cell imaging (BSL2 and 3)
  • Primary cell cultures (human, mouse; macrophages, neutrophils, dendritic cells, lymphocytes, lung epithelial cells)
  • Biofilmassays
  • Subcellular fractionation, phagosome isolation
  • Cell death assays
  • siRNA-based knock down

    
Biochemistry

  • SDS-PAGE, western blot
  • Liposome technology
  • Glycolipid isolation, labeling
  • Thin layer chromatography