Priority Research Area Infections
Mission Projects Funding Techniques Publications Staff
We combine our infection model for tuberculosis with different infection models for malaria in order to investigate the mutual influence of both diseases in the coinfected host.
- Infection of experimental mice with Mycobacterium tuberculosis by aerosol infection (natural route).
- Infection of mice with the rodent malaria parasites Plasmodium berghei or Plasmodium yoelii:
- Sporozoite infection via natural by bite transmission or intravenous injection of isolated sporozoites.
- Blood stage infection via intraperitoneal injection of Plasmodium- infected red blood cells.
In vitro infection models:
Infection of primary macrophages with M. tuberculosis or M. bovis BCG and Plasmodium-infected red blood cells
- Bronchoalveolar lavage (BAL)
- Isolation of organs and blood from experimental mice
- Determining mycobacterial loads in organs or macrophage cultures via colony forming units (CFU)
- Determining parasitemia in Giemsa-stained blood smears
- Analysis of the integrity of the blood-brain-barrier by Evans Blue
- (Immuno-) histochemistry
- Cytometric bead arrays (CBA) and ELISA to determine cytokines and chemokines in biological samples
- Nitric oxide assays
- RNA isolation, cDNA synthesis, quantitative RT-PCR
- Isolation and differentiation of primary mouse cells (bone marrow-derived, peritoneal- and alveolar macrophgages, dendritic cells, T cells)
- Magnetic Cell Separation (MACS) for the purification of different cell populations or Plasmodium-infected red blood cells
- Functional T cell analysis (in vitro restimulation, CFSE-based proliferation assays in vitro and in vivo)
- Flow cytometry
- Fluorescence microscopy
- Confocal microscopy
- Live cell imaging