Clinical and Molecular Allergology
The Division of Clinical and Molecular Allergology is working on the elucidation of sensitization routes and the pathomechanisms of allergy and asthma. In order to achieve these goals we identify allergenic (glyco-/lipo-) proteins and epitopes in allergen sources (inhalant allergens, foods, and biological drugs), isolate and structurally characterize them. These well-characterized molecules are used to improve allergy diagnostic tests, a prerequisite for patient-tailored diagnostics. They are also used for investigating the influence of allergen structures on allergy / asthma development and on the severity and organ-manifestations of allergic symptoms. Because of the close association of the research group with the Interdisciplinary Allergy Outpatient Clinic at the University Hospital Schleswig-Holstein, UKSH, University of Lübeck, (A) the patients are provided with the latest research results (translation of basic science), and (B) basic science is performed with primary material from well-characterized patients in studies with ethical approval.
In the course of the projects, marker allergens will be identified that will allow to determine the individual risk of patients a.) to develop allergy and/or asthma, and b) in the case of already existing allergy to predict the severity of a reaction, the exacerbation of a pre-existing bronchial asthma, and (c) to choose the most beneficial treatment option for the individual patient in the future. Our recent findings regarding structure and function additionally are the basis for the development of novel treatment options.
General Introduction
Allergies are complex multi-organ diseases. In Germany, 12.3 Mio people are affected by allergy alone (asthma not included), and the prevalence is still increasing. Therefore, allergies nowadays are a widespread disease. Some allergies are life-threatening and/or pave the way for chronic diseases such as bronchial asthma as a sequel of a pre-existing allergic rhinitis (e.g. hay fever). These respiratory diseases are recurrent (like rhinitis), are prone to become a chronic illness, and affect the quality of life and overall activity. As a consequence, they lead to major socio-economic burden (Weißbuch Allergologie in Deutschland 2018).
Allergy is an immune reaction to substances of natural origin (from the natural environment) that are as such harmless. Allergies are partly driven by both, a genetic predisposition and environmental factors.
The Research Group Clinical and Molecular Allergology investigates the influence of allergen structure on sensitization/sensitization routes, the development of allergy and asthma, on the grade of symptom severity as well as on localization (lung, gastrointestinal tract, etc.) of allergic symptoms (Molecular Phenotyping).
Projects of the RG Clinical and Molecular Allergology
1. Identification and Characterization of Novel Allergens (CARE)
The identification of new allergens is a main focus of our research and shall lead to the establishment of biomarkers for clinical diagnostics and organ manifestation. Characterization of proteins in terms of structure, molecular weight, hydrophilic/lipophilic characteristics, function (e.g. protease activity) and binding to IgE antibodies from patients’ sera shall help to answer the question: What makes an allergen an allergen? What are the relevant adjuvant factors?
So far, the Research Group (RG) identified and isolated single allergens from plant sources which serve as marker allergens and seem to be associated with the severity of allergic symptoms. The identified allergens are: Cor a 8 (lipid transfer protein of hazelnut), Ara h 6 (storage protein of peanut), Ara h 9 (lipid transfer protein of peanut) as well as in 2015, Ara h 12 and Ara h 13 (peanut defensins) and Ara h 14 and Ara h 15 (peanut oleosins).
Investigations of blood from patients with mild and severe peanut allergy recently revealed that only those suffering from severe peanut allergy had IgE-antibodies against the oleosins. This points to their potential as marker allergens for symptom severity.
The determination of the interactions of allergens with the immune system and the induction of a pro-allergic immune response with resulting IgE antibody synthesis is achieved by partly cell-based structure-function analysis.
The isolated and characterized allergens are important tools for
- the elucidation of sensitization routes (CONTROL)
- the elucidation of disease development (CONTROL)
- the identification of biomarkers which shall allow for the improvement of diagnostic tests, treatment options as well as treatment management (CARE)
2. Investigation of Sensitization Routes (CONTROL)
Sensitization mechanisms of certain allergens and the development of allergic diseases associated with the commonly known symptoms are rarely investigated and not fully understood. In our hands, peanut allergy serves as a model disease for allergy research: Peanut allergy affects several organ systems including the respiratory tract. It is one of the most severe allergic diseases and can sometimes be fatal.
In terms of food allergies, in principle sensitization can occur at the interfaces such as a) the gastrointestinal tract (orally, also via breast feeding), b) the respiratory tract, c) the skin or even earlier d) in utero before birth. One of the main questions of allergology is if peanut exposure during breast feeding may act as a sensitization route or a vector that leads to tolerance induction to peanuts very early in life. Thus, one of our research topics is the investigation on the transfer of peanut allergens into breast milk.
Recently, we could show that marker allergens from peanut, the storage proteins Ara h 2 and Ara h 6 are transferred into breast milk.
Several single peanut allergens have been identified and isolated in the RG over the years.
Some peanut allergens are very stable molecules and can be transferred via air directly inducing bronchospasm after opening a bag with peanut snack. These single allergens shall now be used as tools to elucidate sensitization and elicitation mechanisms of allergy and asthma in projects funded by the German Center for Lung Research (DZL).
A special focus of our research lies on the interaction of lipophilic allergens and the lipids of the respective allergen source and its effect on allergenicity. In addition, we investigate whether lipophilic allergens are preferentially responsible for severe allergic reactions and asthma development and try to elucidate the various underlying mechanisms (Flagship Project Basic Science of the DZL, coordinator: Prof. Uta Jappe).
Apart from peanut as one of the most severe food allergies we purify single allergens from house dust mite and/or produce them recombinantly in order to determine individual sensitization profiles. We recently succeeded in the development of a serum-saving array for the detection of IgE-antibodies against several new house dust mite allergens.
These assays will enable us to identify those allergens that most probably act as “initiator allergens” in sera of very small children from atopic families (DZL-ALLIANCE-cohort) that are responsible for the primary sensitization.
After completion of the identification of these initiator allergens in the childrens’ cohort as well as those mite allergens, which are associated with a risk to develop ashma we would have in our hands a “cocktail” of clinically relevant single allergens which could be investigated for its potential to become an allergy vaccine rather than a component-based specific immunotherapy (Figure ).
3. Determination of Disease Development Mechanisms (CONTROL)
For the determination of the pathomechanism of immediate-type (type I) allergy it is crucial to elucidate how the individual production of allergy-mediating IgE antibodies is triggered by single allergens. Therefore, the specific interaction of allergens at interfaces (surface epithelium at the respiratory and gastrointestinal tract) is examined.
Our aim is to elucidate how allergens are processed and metabolized and if differences exist between allergic and non-allergic individuals. Moreover, we are examining potential disturbances during immune regulation which lead to sensitization and consequently to the clinically relevant allergic reaction. Determination of allergy pathomechanisms including the relevant adjuvant factors is the rational way to battle against allergic diseases.
The group was able to show that different sensitisation profiles are present in patients with different phenotypes of house dust mite allergy. Corresponding allergens were produced recombinantly in Eschericha coli and purified. Using a multiplex assay, sera from patients were analysed with regard to IgE specific for single allergens. The sera from patients with different phenotypes (rhinitis, allergic asthma, and atopic dermatitis) showed different patterns of sensitisation to the allergens. In mild disease (rhinitis), sensitisation to a small number of allergens was detected. In asthma patients, the sensitization pattern was much broader, the "sensitization cout" much higher. There were clear differences for Der p 5 and p 21 IgE levels when compared to the other allergens, so that these can be assumed to be marker allergens for house dust mite-associated asthma. In contrast, significantly more sensitisation to house dust mite allergens was observed in atopic dermatitis. In addition to the known Der p 5 and Der p 21, Der 10, Der p 13 and Der p 20 can be mentioned here.
Based on these results, organ specificity in the recognition of house dust mite allergens was hypothesised. In collaboration with the Hannover Medical School, this question is being investigated as part of the DFG project JA 1007/4-1.
4. Identification and Characterization of Biomarkers for Diagnosis and Therapy (CARE)
The identification of biomarkers in sera of allergic patients is another important field of research of our group. These biomarkers are, for example, a) metabolized and/or immunologically altered allergens, b) allergen-coupled carrier molecules, c) antibodies of the IgE isotype, but also d) other antibody isotypes like allergen-specific IgG and IgA, which may have anti-allergic effects. Here, certain IgE-binding motifs may serve as risk factors for the severity of clinical allergy symptoms. Also a certain allergen-specific IgE-antibody pattern (sensitization profile) may present a risk factor, e.g. a peanut-allergic patient with IgE against the storage proteins Ara h 1, 2 and 3 (see figure, panel 4) as well as the oleosins Ara h 14 and Ara h 15..
Determination of IgE antibodies against single allergens is a molecule-specific diagnosis, and its significance is strongly dependent on the allergen source and the clinical characterization of the single allergens.
This is only possible due to the close connection of the Research Group to the Interdisciplinary Allergy Outpatient Clinic at the University Hospital Schleswig-Holstein (UKSH), University of Lübeck (UzL), both headed by Prof. Dr. med. Uta Jappe.
he identification and characterization of clinically relevant single allergens, cloning and production of recombinant allergens as well as synthesis of peptides which are part of IgE epitopes puts us in the position to perform component-resolved diagnostics. This way, IgE antibodies specific for an allergenic protein or a certain sequential epitope can be identified and analyzed. Thus, individual sensitization patterns (“allergograms”) against different a) proteins, e.g. food allergens like peanut and lupine, house dust mite allergens, pollen, drugs like novel biological drugs, b) homologous (structurally related) proteins in different food and c) different antibody-binding motifs (IgE binding regions) of a single allergen are determined (precision diagnostics).
During the last years, we succeeded in the isolation of marker allergens for allergy severity from peanut, the oleosins. Since these allergens are not soluble in aqueous solutions, they are most probably not part of the solutions provided presently for routine allergy diagnostic tests or treatments.
At present we are working at the transfer of these lipophilic allergens into routine allergy diagnostic tests (transfer) and investigate their potential as therapeutic allergens and the according application routes.
Additionally, we have developed a multianalyte assay for the detection of delayed anaphylaxis to meat, a new entity called “the alpha-GAL syndrome”, which is already in use for the patients in the RG-associated allergy outpatient clinics. In some cases in which the routine diagnostic tests were negative, our multianalyte assay led to the correct diagnosis and therefore enabled the patients to re-evaluate their diet and reduce their risk of anaphylaxis (precision diagnostics).
In our project on immune reactions to target treatments (biologicals) we identified single epitopes which are most probably responsible for the loss of efficacy during treatment due to anti-drug-antibodies in patients’ sera (Proof-of-Principle).
We investigate sera from patients with hypersensitivity reactions to biologicals in order to develop assays that will allow the monitoring of anti-drug-antibodies during biological treatment and the switch to safe and effective alternative biologicals in the near future (individualized diagnostic, precision diagnostic).
ImproviNg DIagnostiCs And ThErapy of Food Hypersensitivity (INDICATE-FH): a new BMBF-financed consortium
Recently, we started a new BMBF-financed project called "ImproviNg DIagnostiCs And ThErapy of Food Hypersensitivity" (INDICATE-FH) in a consortium with the Institute of Nutritional Medicine of the University of Lübeck and the University of Hohenheim to improve diagnostics regarding intolerance to wheat. Food intolerances to wheat flour are common problems in medical practice. In many cases, the non-specific gastrointestinal symptoms are misinterpreted as irritable bowel syndrome. The main reason for this is the lack of specific biomarkers and practical diagnostic procedures. The main goal of the project is to optimize existing diagnostics for non-celiac wheat sensitivity (NCWS) in differentiation to celiac disease (CD), wheat allergy (WA) and irritable bowel syndrome. The FG Clinical and Molecular Allergology works on the pathogenesis of wheat intolerance and improvement of diagnostics for wheat allergy. Allergenic proteins from wheat and lupine are identified, purified, characterized and recombinantly produced to improve the sensitivity and specificity of allergy tests. Using these single allergens, antibody- and cell-based assays will be developed and optimized with the goal of more reliably distinguishing WA from CD and NCWS as well as intestinal diseases of other origin in the future. In addition, the individual allergens will be investigated to determine whether they are associated with the severity of an allergic reaction in the sense of a biomarker. Furthermore, transcriptome and methylome analyses will be used to screen for candidate genes with biomarker potential that could in the future allow discrimination between allergic and non-allergic reactions to wheat using a simple blood test (Prof. T. Goldmann, Borstel). The recombinant single allergens of wheat as well as the newly identified allergens of lupine will be used for mechanistic pathogenesis elucidation. This will optimize the diagnosis and –eventually- the treatment of patients with symptoms to wheat products, which will be a relief for many patients as they will be able to change their diet accordingly to get through their daily life as symptom-free as possible.
5. Setup of Registries and Data Bases
Data which was acquired in registries and data bases is used for epidemiologic studies and serves as a tool for the detection of new allergies. Furthermore, the Research Group is involved in the setup of the infrastructure for and participates in biomaterial collection BioMaterialBank (BMB) Nord.
Additional Links
Collaborative projects
- German Center for Lung Research (DZL)
- Airway Research Center North (ARCN)
- Cluster of Excellence
- INDICATE-FH (BMBF-Verbundprojekt)
Clinics
- Interdisciplinary Outpationt Clinic, Dept. of Pneumology, University Hospital Schleswig-Holstein (UKSH), University of Lübeck (UzL)
German Research Foundation
- DFG JA 1007/4-1: The role of house dust mite single allergens - structural and functional features in the context of atopic diseases 2023-2026.
- DFG JA 1007/2-3: Investigation of peanut oil bodies and the new lipophilic allergens like oleosins regarding promotion of sensitization and elicitation processes in food allergy (2019-2022)
- DFG JA1007/2-1: Structural- and functional investigations of lipophilic peanut allergens with special emphasis on oleosins (2013-2015)
- DFG Scho 828/2-1: Investigation of peanut antigen /peanut allergens in breast milk after having been processed at mucosal interfaces and their interaction with the immune system
Exzellenz Cluster: 2019 - 2025 Precision Medicine in Chronic Infammation (PMI)
Federal Ministry of Education and Science
- FKZ:01EA2109B Verbundprojekt ImproviNg DIagnostiCs And ThErapy of Food Hypersensitivity; INDICATE-FH (PI: U. Jappe) (2021-2024)
- DZL: Disease Area Asthma & Allergy (DA-AA), German Center for Lung Research (DZL) (PI U. Jappe); AA-FPB: Co-ordinator of the Flagship Project Basic Science: U. Jappe) (2010-2027)
- DZL - Flexible Funds; Single Cell Analysis (SCA): Cell Circuits in Predictive and Personalized Pulmonary Medicine. PI Prof. Uta Jappe, cooperation with Prof. Marc Ehlers, UzL (2024-2027)
- FK 01KG 0911 Verbundprojekt Birch Associated Soy Allergy and Immuno-Therapy (BASALIT) (PI. U. Jappe) (2009-2015)
Federal Ministry of Economy and Technology
- ZIM-KF2784702SB4: Förderprogramm Zentrales Innovationsprogramm Mittelstand (ZIM), Fördermodul Kooperationsprojekte: Development of an innovative test to prevent secondary treatment failure due to hypersensitivity reaction to a target treatment with biologicals (PI: Uta Jappe) (2015-2018)
- ZIM-KF2784701AJ0: Förderprogramm Zentrales Innovationsprogramm Mittelstand (ZIM), Fördermodul Kooperationsprojekte: Development of tests for the detection of specific IgE-antibodies directed against therapeutic monoclonal antibodies (biologicals) (PI: Uta Jappe) (2011-2015)
Federal Ministry of Food and Agriculture (BMEL)
- Federal Ministry of Food and Agriculture (BMEL) via the Federal Office for Agriculture and Food (BLE) under the innovation support programme "Development of an aptamer-based biosensor for the detection and investigation of allergens and their allergenic potential in foods; AptaSens" (2020-2023)
Foundations
- Kanert-Foundation: Investigation of peanut oilbodies for allergen transport with subsequent analysis of reconstituted oil bodies as vehicles for therapeutic allergen transport (epicutaneous immunotherapy (EPIT)) for peanut allergy (PI. U. Jappe) (2018-2019)
EU - 7. FP
- FP7 RegPot 2010-5_256756FCUB Er Scientific Support of the West Balkan states; cooperation with the Dept. of Biochemistry, University of Belgrade, Serbia
Member of the German Center for Lung Research (DZL) and Airway Research Center North (ARCN)
Member of Companion Diagnostics Network
Member of Network of Severe Allergic Reactions (NORA)
Genetic Methods
- Cloning and recombinant expression of allergens
- E. coli, "Clear coli"
- Pichia pastoris
- Insect cells (Sf9 cells)
- RNA-silencing of specific genes
- (real-time) PCR
Allergen/Protein Purification
- Aqueous and organic extraction methods
- Chromatography
- Affinity chromatography
- Ion exchange
- Hydrophobic interaction
- Reversed phase
- (Preparative) gel electrophoresis
- purification of lipophilic allergens
Allergen/Protein Analysis
- Electrophoresis
- Isoelectric focusing (IEF)
- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- 2D-SDS-PAGE
- Difference gel electrophoresis (DIGE)
- Immunoblotting
- Detection of IgE, IgG und IgG subclasses
- Colorimetric readout
- Readout by chemiluminescence
- ELISA Techniques and ELISA establishment
- N-terminal sequencing
- Zymography
- Enzyme kinetics
- Epitope mapping of allergens
Design of peptide arrays
Mapping of b cells epitopes: IgE / IgG
Allergen/Protein engineering
- Protein fragmentation by protease digestion
- simulation of human digestion (Enzynorm/Kreon)
- Protein analysis: reduction / native analysis
- Controlled deglycosylation of proteins
- Enzymatic: Glycosidases
- Chemical: Periodate
Cell analysis / cell culture
- Cultivation of human / mammalian cells
- Cell analysis by flow cytometry (FCM, FACS)
- Analysis of cell activation by allergens (BAT, basophile activation test)
- Fluorescence microscopy, FRET techniques
Further techniques
- Generation of monoclonal antibodies and antisera (MAB technique)
- Immunohistochemistry (IHC)
- In silico protein-ligand docking studies (Autodock)
Allergie-Kolloquium Borstel
- 23. Allergie-Kolloquium 2010 - (see details)
- 24. Allergie-Kolloquium 2011 - (see details)
- 25. Allergie-Kolloquium 2012 - (see details)
- 26. Allergie-Kolloquium 2013 - (see details)
- 27. Allergie-Kolloquium 2014 - (see details)
- 28. Allergie-Kolloquium 2015 - (see details)
- 29. Allergie-Kolloquium 2016 - (see details)
- 30. Allergie-Kolloquium 2017 - (see details)
- 31. Allergie-Kolloquium 2018 - (see details)
- 32. Allergie-Kolloquium 2019 - (see details)
- 33. Allergie-Kolloquium 2021 - (see details)
- 34. Allergie-Kolloquium 2022 - (see details)
- 35. Allergie-Kolloquium 2023 - (see details)
- 36. Allergie-Kolloquium 2024 - (see details)
Lübecker Allergie-Symposium
- 1. Lübecker Allergiesymposium 2010 - (see details)
- 2. Lübecker Allergiesymposium 2011 - (see details)
- 3. Lübecker Allergiesymposium 2012 - (see details)
- 4. Lübecker Allergiesymposium 2013 - (see details)
- 5. Lübecker Allergiesymposium 2015 - (see details)
Internationaler Kongress
7th International Symposium on Molecular Allergology (ISMA), Luxembourg 2017 (see details)
International Workshop on Molecular Allergology
Joint workshop of the WHO/IUIS Allergen Nomenclature Subcommittee and the EAACI Task Force BACALL, Borstel 2023
DGAKI-Symposium
Allergieakademie der DGAKI 2019 (see details)
44th Symposium of the North German Immunologists
NDI3 - New Developments in Immunology, Inflammation and Infection, 2021 (see details)
2006: Award of the German Contact Allergy Research Group (DKG) (Uta Jappe and co-authors)
2006: Deutsche Gesellschaft für Ultraschall in der Medizin (DEGUM) Scientific Award (Uta Jappe and co-authors)
2007: Kanert Price for Allergy Research (Wolf-Meinhard Becker und Arnd Petersen)
2010: Poster Prize of the European Academy of Allergy and Clinical Immunology (EAACI) Congress in London, Great Britain (Uta Jappe and co-authors)
2011: Abstract Prize „Best of Mainz“, Scientific Symposium of the DGAKI in Mainz („Mainzer Allergie-Workshop”) (Uta Jappe)
2011: Best Master-Thesis-Award of the Research Center Borstel in 2011 to Saskia Hellmig; Title of the Thesis: “Lupine as cause of food allergy: Allergen-identification and characterization”.
2012: Poster Award, XX. North-German Society of Dermatology in Berlin (Uta Jappe and co-authors)
2012: Award for the Best Talk, European Academy of Allergy and Clinical Immunology (EAACI) Congress in Geneva, Switzerland (Uta Jappe)
2013: Award for the Best Talk, European Academy of Allergy and Clinical Immunology (EAACI) and the World Allergy Organization (WAO) in Milano, Italy (Uta Jappe)
2015: Best Master-Thesis-Award of the Research Center Borstel in 2015 to Alexandra Scharf; Title of the Thesis: “Development and validation of an ELISA for the quantification of the peanut allergen Ara h 2 in breast milk.”
2015: Award for the Best Talk, 38th Symposium of North German Immunologists, 2015 in Borstel, Germany (Christian Schwager)
2016: Best contribution (Talk and Poster) on the Retreat of the Cluster of Excellence „Inflammation at Interfaces“ (Arne Homann and co-authors)
2016: Travel Grant and Biolegend Best Talk Award, X. World Immune Regulation Meeting (WIRM), Davos, Switzerland (Arne Homann).
2016: Best Master-Thesis-Award of the Research Center Borstel in 2016 to Arabella Karstedt; Title of the Thesis: "Isolation and characterization of new lupine allergens".
2017: Young Talent Award of the German Society for Allergy and Clinical Immunology (DGAKI) for the most valuable Publication (Christian Schwager for all authors)
2018: Kanert Price for Allergy Research (Uta Jappe and Christian Schwager)
2018: District-Segeberg-Award for the best PhD thesis of the Research Center Borstel (Christian Schwager) Title of the thesis: Peanut oleosins: isolation, characterization and demonstration of their clinical relevance”
2020: Best Master-Thesis-Award of the Research Center Borstel in 2020 to Vivian Valeska Lelleck; Title of the Thesis: "Lipophilic peanut allergens for allergy diagnostic tests".
2022: 2nd Abstract Prize of the DGAKI on the Mainzer Allergie-Workshop 2022 for the cooperative project between Research Center Borstel and the Max-Rubner-Institutes Kiel and Karlsruhe to the authors Roggensack T, Rodriguez Gomez M, Hui-Zhi-Low, Kappel K, Uta Jappe, Clawin-Rädecker I.
2022: Best Master-Thesis-Award of the Research Center Borstel (RCB) in 2022: Title: "Optimization and validation of the heterologous expression of a food allergen belonging to the group of lipid transfer proteins" (Elisabeth Marie Albert)
2023: Award for the Best Talk (Flash Talk),Congress of the European Academy of Allergy and Clinical Immunology (EAACI) in Hamburg (Theresa Walsemann)
2023: Award for the Best Poster Presentation (Thematic Poster Session), Congress of the European Academy of Allergy and Clinical Immunology (EAACI) in Hamburg (Marua Abu Risha)
2023: District-Segeberg-Award for the best PhD thesis of the Research Center Borstel (Theresa Walsemann) Title of the thesis: "Identification and characterization of house dust mite allergens influencing the allergic phenotype and improvement of allergy diagnosis in clinical routine."
2024: Travel Grant of the European Academy of Allergy and Clinical Immunology (EAACI), EAACI Congress in Valencia, Spain (Marua Abu Risha)
2024: Travel Grant Award to Marua Abu Risha for the Congress of the European Academy of Allergy and Clinical Immunology (EAACI) in Valencia, Spain
Press
2022: Prof. Dr. Uta Jappe has been continuously listed in Focus-Gesundheit among Germany's top physicians from 122 specialties since 2017
2023 and 2024: Prof Dr. Uta Jappe is one of the excellent specialists in the STERN Doctors List 2024 in the area of food intolerances.
2024
Abu Risha, M, Reddy, KD, Nemani, SSP, Jakwerth, C, Schmidt-Weber, C, Bahmer, T, Hansen, G, von Mutius, E, Rabe, KF & Dittrich, A-M et al. 2024, 'Epigenetic training of human bronchial epithelium cells by repeated rhinovirus infections', ALLERGY, Jg. 79, Nr. 12, S. 3385-3400. https://doi.org/10.1111/all.16388
Abu Risha, M, Rick, E-M, Plum, M & Jappe, U 2024, 'Legume Allergens Pea, Chickpea, Lentil, Lupine and Beyond', Current Allergy and Asthma Reports, Jg. 24, Nr. 9, S. 527-548. https://doi.org/10.1007/s11882-024-01165-7
Darsow, U, Gelincik, A, Jappe, U, Platts-Mills, TA, Ünal, D & Biedermann, T 2024, 'Algorithms in allergy: An algorithm for alpha-Gal syndrome diagnosis and treatment, 2024 update', ALLERGY, Jg. 79, Nr. 11, S. 3169-3172. https://doi.org/10.1111/all.16291
Jappe, U, Bergmann, K-C, Brinkmann, F, Faihs, V, Gülsen, A, Klimek, L, Renz, H, Seurig, S, Taube, C, Traidl, S, Treudler, R, Wagenmann, M, Werfel, T, Worm, M & Zuberbier, T 2024, 'Biologics in allergology and clinical immunology: Update on therapies for atopic diseases, urticaria, and angioedema and on safety aspects focusing on hypersensitivity reactions', Allergologie select, Jg. 8, S. 365-406. https://doi.org/10.5414/ALX02533E
Pascal, M, Bax, HJ, Bergmann, C, Bianchini, R, Castells, M, Chauhan, J, De Las Vecillas, L, Hartmann, K, Álvarez, EI & Jappe, U et al. 2024, 'Granulocytes and mast cells in AllergoOncology-Bridging allergy to cancer: An EAACI position paper', ALLERGY, Jg. 79, Nr. 9, S. 2319-2345. https://doi.org/10.1111/all.16246
Rick, E-M, Woolnough, K, Richardson, M, Monteiro, W, Craner, M, Bourne, M, Cousins, DJ, Swoboda, I, Wardlaw, AJ & Pashley, CH 2024, 'Identification of allergens from Aspergillus fumigatus-Potential association with lung damage in asthma', ALLERGY, Jg. 79, Nr. 5, S. 1208-1218. https://doi.org/10.1111/all.16032
Treudler, R, Worm, M, Bauer, A, Dickel, H, Heine, G, Jappe, U, Klimek, L, Raulf, M, Wedi, B, Wieczorek, D, Francuzik, W, Jakob, T, Pfaar, O, Ring, J, Rueff, F, Schnadt, S, Werfel, T, Wurpts, G, Zarnowski, J, Zuberbier, T & Brockow, K 2024, 'Occupational anaphylaxis: A Position Paper of the German Society of Allergology and Clinical Immunology (DGAKI)', Allergologie select, Jg. 8, S. 407-424. https://doi.org/10.5414/ALX02543E
Turner, MC, Radzikowska, U, Ferastraoaru, DE, Pascal, M, Wesseling, P, McCraw, A, Backes, C, Bax, HJ, Bergmann, C & Bianchini, R et al. 2024, 'AllergoOncology: Biomarkers and refined classification for research in the allergy and glioma nexus-A joint EAACI-EANO position paper', ALLERGY, Jg. 79, Nr. 6, S. 1419-1439. https://doi.org/10.1111/all.15994
Buchbeitrag / Book contribution
Dramburg S, Hilger C, Santos AF, de Las Vecillas L, Aalberse RC, Acevedo N, Aglas L, Altmann F, Arruda KL, Asero R, Ballmer-Weber B, Barber D, Beyer K, Biedermann T, Bilo MB, Blank S, Bosshard PP, Breiteneder H, Brough HA, Bublin M, Campbell D, Caraballo L, Caubet JC, Celi G, Chapman MD, Chruszcz M, Custovic A, Czolk R, Davies J, Douladiris N, Eberlein B, Ebisawa M, Ehlers A, Eigenmann P, Gadermaier G, Giovannini M, Gomez F, Grohman R, Guillet C, Hafner C, Hamilton RG, Hauser M, Hawranek T, Hoffmann HJ, Holzhauser T, Iizuka T, Jacquet A, Jakob T, Janssen-Weets B, Jappe U, Jutel M, Kalic T, Kamath S, Kespohl S, Kleine-Tebbe J, Knol E, Knulst A, Konradsen JR, Korošec P, Kuehn A, Lack G, Le TM, Lopata A, Luengo O, Mäkelä M, Marra AM, Mills C, Morisset M, Muraro A, Nowak-Wegrzyn A, Nugraha R, Ollert M, Palosuo K, Pastorello EA, Patil SU, Platts-Mills T, Pomés A, Poncet P, Potapova E, Poulsen LK, Radauer C, Radulovic S, Raulf M, Rougé P, Sastre J, Sato S, Scala E, Schmid JM, Schmid-Grendelmeier P, Schrama D, Sénéchal H, Traidl-Hoffmann C, Valverde-Monge M, van Hage M, van Ree R, Verhoeckx K, Vieths S, Wickman M, Zakzuk J, Matricardi PM, Hoffmann-Sommergruber K. Molecular Allergology Pocket Guide. European Academy of Allergy and Clinical Immunology & John Wiley and sons Ltd. 2024
2023
Albert E, Walsemann T, Behrends J, Jappe U. Lipid transfer protein syndrome in a Northern European patient: An unusual case report. Front Med (Lausanne). 2023 Feb 7;10:1049477. doi: 10.3389/fmed.2023.1049477. eCollection 2023.
Dölle-Bierke S, Höfer V, Francuzik W, Näher F, Bilo M.B., Cichocka-Jarosz E, Felipe Ensina L, Fernandez-Rivas M, García Figueroa B, Hartmann K, Jappe U, Köhli A, Lange L, Maris I, Mustakov T, Nemat K, Ott H, Papadopoulos NG, Pföhler C, Ruëff F, Spindler T, Stock P, Treudler R, Vogelberg C, Wagner N, Worm M. Food-induced anaphylaxis: Data from the European Anaphylaxis Registry. J Allergy Clin Immunol Pract. 2023 Mar 27:S2213-2198(23)00312-4. doi: 10.1016/j.jaip.2023.03.026. Online ahead of print.
Dramburg S, Hilger C, Santos AF, de las Vecillas L, Aalberse RC, Acevedo N, Aglas L, Altmann F, Arruda KL, Asero R, Ballmer-Weber B, Barber D, Beyer K, Biedermann T, Bilo MB, Blank S, Bosshard PP, Breiteneder H, Brough HA, Bublin M, Campbell D, Caraballo L, Caubet JC, Celi G, Chapman MD, Chruszcz M, Custovic A, Czolk R, Davies J, Douladiris N, Eberlein B, Ebisawa M, Ehlers A, Eigenmann P, Gadermaier G, Giovannini M, Gomez F, Grohman R, Guillet C, Hafner C, Hamilton RG, Hauser M, Hawranek T, Hoffmann HJ, Holzhauser T, Iizuka T, Jacquet A, Jakob T, Janssen-Weets B, Jappe U, Jutel M, Kalic T, Kamath S, Kespohl S, Kleine-Tebbe J, Knol E, Knulst A, Konradsen JR, Korošec P, Kuehn A, Lack G, Thuy-My Le, Lopata A, Luengo O, Mäkelä M, Marra AM, Mills C, Morisset M, Muraro A, Nowak-Wegrzyn A, Nugraha R, Ollert M, Palosuo K, Pastorello EA, Patil SU, Platts-Mills TA, Pomés A, Poncet P, Potapova E, Poulsen LK, Radauer C, Radulovic S, Raulf M, Rougé P, Sastre J, Sato S, Scala E, Schmid JM, Schmid-Grendelmeier P, Schrama D, Sénéchal H, Traidl-Hoffmann C, Valverde-Monge M, van Hage M, van Ree R, Verhoeckx K, Vieths S, Wickman M, Zakzuk J, Matricardi PM, Hoffmann-Sommergruber K. EAACI Molecular Allergology User's Guide 2.0. First published: 26 April 2023 https://doi.org/10.1111/pai.13854
Dühring, L, Petry, J, Lilienthal, G-M, Bartsch, YC, Kubiak, M, Pfeufer, C, Lehrian, S, Buhre, JS, Lunding, HB, Kern, C, Behrends, J, Walsemann, T, Gädert, L, Sommer, C, Krüger, L, Blanchard, V, Dehmel, S, Jappe, U, Rahmöller, J & Ehlers, M 2023, 'Sialylation of IgE reduces FcεRIα interaction and mast cell and basophil activation in vitro and increases IgE half-life in vivo', ALLERGY, Jg. 78, Nr. 8, S. 2301-2305. https://doi.org/10.1111/all.15665
Đurašinović, T, Lopandić, Z, Protić-Rosić, I, Nešić, A, Trbojević-Ivić, J, Jappe, U & Gavrović-Jankulović, M 2024, 'Identification of S-adenosyl-l-homocysteine hydrolase from banana fruit as a novel plant panallergen', Food chemistry, Jg. 437, Nr. Pt 1, S. 137782. https://doi.org/10.1016/j.foodchem.2023.137782
Heßler N, Kordowski A, Sasse J, Ahlemann G, Schulz F, Schröder T, Exner A, Jablonski L, Jappe U, Bischoff SC, Grzegorzek M, König IR, Sina C. Study protocol to investigate the efficacy of confocal laser endomicroscopy-based selective single-elimination diet over standard fivefold elimination diet in patients with endomicroscopically proven food intolerance: app-assisted, monocentric, double-blind, randomised and controlled trial in Germany.BMJ Open. 2023 Nov 2;13(11):e072024. doi: 10.1136/bmjopen-2023-072024.
Jappe U. Vegan diet - alternative protein sources as potential allergy risk. Allergo J Int 2023; 32:251-257 doi.org/10.1007/s40629-023-00248-7
Jappe, U. Das Alpha-Gal-Syndrom – eine komplexe Allergie im interdisziplinären Kontext. Aktuelle Ernährungsmedizin 2023; 48(03): 195-208. DOI: 10.1055/a-1870-7328
Plum M, Tjerrild L, Raiber T, Bantleon F, Bantleon S, Miehe M, Jabs F, Seismann H, Möbs C, Pfützner W, Jakob T, Andersen GR, Spillner E. Structural and functional analyses of antibodies specific for modified core N-glycans suggest a role in TH 2 responses. Allergy. 2023;78(1):121-130. doi: 10.1111/all.15417.
Vegan diets from an allergy point of view - Position paper of the DGAKI working group on food allergy. Allergol Select. 2023 Mar 31;7:57-83. doi: 10.5414/ALX02400E. eCollection 2023
Walsemann T, Böttger M, Traidl S, Schwager C, Gülsen A, Freimooser S, Roesner LM, Werfel T, Jappe U. Specific IgE against the house dust mite allergens Der p 5, 20 and 21 influences the phenotype and severity of atopic diseases. Allergy. 2023;78(3):731-742. doi: 10.1111/all.15553
2022
Jappe U. Oral Allergy Syndrome. In: Diseases of the Oral Mucosa; E. Schmidt (ed.), Springer Verlag 2022; Chapter 40; pp 427-434.
Kasper B, Yue X, Goldmann T, Gülsen A, Kugler C, Yu X, Petersen F (2022) Air exposure and cell differentiation are essential for investigation of SARS-CoV-2 entry genes in human primary airway epithelial cells in vitro. Frontiers in Medicine 9: 897695 (ARCN). 2022. Link zum Artikel
Kleine-Tebbe J, Brans R, Jappe U. Allergene: Auslöser unterschiedlicher Allergie-Varianten. Allergo Journal 2022; 31: 16-32
Schocker F, Jappe U. Breastfeeding: Maternally transferred allergens in breast milk: Protective or sensitizing? Mol Nutr Food Res. 2022 May 26:e2200066. doi: 10.1002/mnfr.202200066. https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202200066
Buchbeitrag (Originalartikel 2021) / Book contribution (original article 2021)
Jappe U, Karstedt A, Warneke D, Hellmig S, Böttger M, Riffelmann FW, Treudler R, Lange L, Abraham S, Dölle-Bierke S, Worm M, Wagner N, Rueff F, Reese G, Knulst AC, Becker WM. Identification and Purification of Novel Low-Molecular-Weight Lupine Allergens as Components for Personalized Diagnostics. In: Food Allergies in Modern Life; eds: Sara Manti, Gian Luigi Marseglia and Salvatore Leonardi. MDPI 2022: 63-83 (www.mdpi.com/journal/nutrients)
Head
Scientific staff
Technical staff
Alumni
- Elisabeth Albert
- Dr. Mareike de Vries
- Dr. med. Askin Gülsen
- MSc Saskia Hellmig
- Dr. Arne Homann
- MSc Arabella Karstedt
- Dr. med. Tamina Kolaly
- Dr. Skadi Kull
- MSc Vivian Lelleck
- Dr. Sandra Minge
- MSc Muriel Mrose
- Carolin Murawski
- Dr. med. Annika Opitz
- Prof. Dr. Arnd Petersen
- Dr. Sandra Rennert
- MSc Alexandra Scharf
- Dr. Frauke Schocker
- Dr. Christian Schwager
- Theresa Walsemann
- Dr. Lizzy Wanka