Central Group

Histology

  Mission    Projects    Funding    Techniques    Publications    Staff

Techniques

We work primarily on histological material. Its processing (FFPE, freeze, HOPE) is our central methodical basis. In addition to conventional histological staining techniques, we frequently and regularly work with immunohistochemical staining methods.

Multispectral Immunhistochemistry and Spatial Transcriptomics

We are delighted to have a multispectral immunohistochemical platform (Akoya Vectra-Polaris) available through funding from the DZL, which, in addition to our other technologies (transcriptome, nanostring, cell culture), decisively expands the methodological portfolio. In addition, we managed to acquire a platform for spatial transcriptomics (10x) together with the research group early childhood asthma.

We are very delighted to have a multispectral immunohistochemical platform (Akoya Vectra-Polaris) through a DZL funding, which decisively expands the methodological portfolio alongside our other technologies (transcriptome, nanostring, cell culture). In addition, we have succeeded in acquiring a platform for Spatial Transcriptomics (10x) together with the reseach group early childhood asthma.

Transcriptome analyses

In addition to transcriptomics on our DZL-financed Agilent platform (incl. Bioanalyzer and Nanodrop), we offer investigations on the Nanostring N-Counter, providing a prep station. We also apply a light cycler.

Cell culture

We use different cell systems to enable functional studies. In addition to cell lines, this also includes human primary cell cultures (in cooperation with the Grosshansdorf Lung Clinic). Appropriate read out systems are western blot, in cell western and fluorescence microscopy.