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Histology

Prof. Dr. rer. nat. Torsten Goldmann
Prof. Dr. rer. nat. Torsten Goldmann
+49 4537 / 188-2310
+49 4537 / 188-2290
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Within the Core Unit, we carry out histological work on animal models generated in FZB projects. In addition to consulting on pre-analysis, we process tissue for embedding in paraffin, preparation of sections, histological staining, immunohistochemistry and archiving of block material. Frozen sections are also part of our tasks. In the field of archieving, we work closely with BMB Nord. We consult the various research groups on the planning and execution of histological analyzes as well as further investigations such as transcriptomics, multispectral IHC, nanostring and spatial transcriptomics.

 

Research

As part of the German Center for Lung Research (DZL) at the North site (ARCN), we are involved in third-party funded projects in the disease areas lung cancer, COPD, asthma/allergy and biobanking. Our particular research focus is the TGF-beta signaling pathway in NSCLC. Moreover, embryological processes and the tumor microenvironment are gaining increasing relevance. After years of basic research, we are now intensively engaged in translating the results obtained in this field into clinical practice. In the area of infection, we are particularly interested in the multispectral immunohistochemical analysis of pathophysiological events in tuberculosis granulomas and transcriptome studies within various model systems and cohorts, with the aim of developing host directed therapies.

 

 

  • DZL LC central project Tumor microenvironment in Lung Cancer
    Cooperation with R. Savai (MPI Bad Nauheim), Univ. Heidelberg (M. Thomas, H. Winther, P. Christopoulos), DKFZ (U. Klingmüller), Lungenclinic Grosshansdorf (M. Reck, K.F Rabe, H. Watz), Univ. Lübeck (D. Drömann). Univ. München (A. Tufmann).
  • DZL-DZIF overarching project: Multispectral Immunohistochemistry, Transcriptomics and Epigenetics in Tuberculosis
    Cooperation with A. Blumenthal (Univ. Queensland, Australia), T. Dallenga, U. Schaible, N. Shubladze (Univ. Tblisi, Georgia) C. Lange, Ch. Hölscher, S. Niemann and N. Reiling.
  • DZL LC basic science flagship project: TGF beta signaling in NSCLC
    Cooperation with MPI Bad Nauheim (R. Savai, Pirfenidone), CPC-München (G. Stathopoulos, SMAD-Linker) und DKFZ (U. Klingmüller) and LungenClinic Grosshansdorf (M.Reck, K.F. Rabe), Univ. Lübeck (D. Drömann).
  • DZL LC translational flagship project: Epigenetics of NSCLC
    Cooperation with Univ. Ulm (O. Ammerpohl), Lungenclinic Grosshansdorf (M. Reck, K.F. Rabe)
  • DZL LC COPD and AA
    Cooperation with LungenClinic (H. Watz, K.F. Rabe), Univ. Ulm (O. Ammerpohl), D. Schwudke, S. Krauss-Etschmann, U. Jappe, S. Meiners, H. Heine, K. Stein, M. Wegmann, F. Petersen, A. Frey, H. Fehrenbach and K. Brandenburg.
  • BMBF Indicate FH
    Histology, Transcriptomics and Methylomes in Wheat Allergy and Hypersensitivity. Cooperation with U. Jappe, Th. Kufer (Univ. Stuttgart), S. Bischoff (Univ. Stuttgart), Christian Sina (Univ. Lübeck).

 

BMBF

  • German Center for Lung Research (DZL), Airway Research Center North (ARCN),
    Disease Area LC (Lung Cancer). Project duration: 1.1. 2021 – 12/31/2023.
  • German Center for Lung Research (DZL), Airway Research Center North (ARCN),
    Disease Area COPD (COPD). Project duration: 1.1. 2021 – 12/31/2023.
  • German Center for Lung Research (DZL), Airway Research Center North (ARCN),
    Platform Biobanking. Project duration: 1.1. 2021 – 12/31/2023.
  • Indicate FH. Project duration: 2022-2025.

 

We work primarily on histological material. Its processing (FFPE, freeze, HOPE) is our central methodical basis. In addition to conventional histological staining techniques, we frequently and regularly work with immunohistochemical staining methods.

 

  • Multispectral Immunhistochemistry and Spatial Transcriptomics
    We are delighted to have a multispectral immunohistochemical platform (Akoya Vectra-Polaris) available through funding from the DZL, which, in addition to our other technologies (transcriptome, nanostring, cell culture), decisively expands the methodological portfolio. In addition, we managed to acquire a platform for spatial transcriptomics (10x) together with the research group early childhood asthma.
    We are very delighted to have a multispectral immunohistochemical platform (Akoya Vectra-Polaris) through a DZL funding, which decisively expands the methodological portfolio alongside our other technologies (transcriptome, nanostring, cell culture). In addition, we have succeeded in acquiring a platform for Spatial Transcriptomics (10x) together with the reseach group early childhood asthma.
  • Transcriptome analyses
    In addition to transcriptomics on our DZL-financed Agilent platform (incl. Bioanalyzer and Nanodrop), we offer investigations on the Nanostring N-Counter, providing a prep station. We also apply a light cycler.
  • Cell culture
    We use different cell systems to enable functional studies. In addition to cell lines, this also includes human primary cell cultures (in cooperation with the Grosshansdorf Lung Clinic). Appropriate read out systems are western blot, in cell western and fluorescence microscopy.

 

2024

Carow, B, Muliadi, V, Skålén, K, Yokota, C, Kathamuthu, GR, Setiabudiawan, TP, Lange, C, Scheu, K, Gaede, KI & Goldmann, T et al. 2024, 'Immune mapping of human tuberculosis and sarcoidosis lung granulomas', FRONTIERS IN IMMUNOLOGY, Jg. 14, S. 1332733. https://doi.org/10.3389/fimmu.2023.1332733

Hertz, D, Eggers, L, von Borstel, L, Goldmann, T, Lotter, H & Schneider, BE 2024, 'Turning the tables: Loss of adaptive immunity reverses sex differences in tuberculosis', Biorxiv, S. 2024.09.24.614709. https://doi.org/10.1101/2024.09.24.614709

Hertz, D, Marwitz, S, Eggers, L, von Borstel, L, Parvathy, GH, Behrends, J, Jonigk, DD, Manz, RA, Goldmann, T & Schneider, BE 2024, 'Ablation of B cell-derived IL-10 increases tuberculosis resistance', Biorxiv, S. 2024.07.17.603865. https://doi.org/10.1101/2024.07.17.603865

Höhne, K, Wagenknecht, A, Maier, C, Engelhard, P, Goldmann, T, Schließmann, SJ, Plönes, T, Trepel, M, Eibel, H & Zissel, G 2024, 'Pro-Fibrotic Effects of CCL18 on Human Lung Fibroblasts Are Mediated via CCR6', Cells, Jg. 13, Nr. 3, 238. https://doi.org/10.3390/cells13030238

Kuempers, C, Schnepf, K, Marwitz, S, Watermann, C, Scheel, A, Fischer, RN, Ammerpohl, O, Perner, S, Drömann, D & Goldmann, T 2024, 'Upregulation and epigenetic modification of the creatine transporter SLC6A8 in non-small cell lung cancer', Histology and histopathology, Jg. 39, Nr. 7, S. 867-876. https://doi.org/10.14670/HH-18-731

Richtmann, S, Marwitz, S, Muley, T, Koistinen, H, Christopoulos, P, Thomas, M, Kazdal, D, Allgäuer, M, Winter, H & Goldmann, T et al. 2024, 'The pregnancy-associated protein glycodelin as a potential sex-specific target for resistance to immunotherapy in non-small cell lung cancer', Translational Research, Jg. 272, S. 177-189. https://doi.org/10.1016/j.trsl.2024.02.007

Scheffzük, C, Biedziak, D, Gisch, N, Goldmann, T & Stamme, C 2024, 'Surfactant protein A modulates neuroinflammation in adult mice upon pulmonary infection', Brain research, Jg. 1840, S. 149108. https://doi.org/10.1016/j.brainres.2024.149108

 

2023

Chen, J, Wang, X, Schmalen, A, Haines, S, Wolff, M, Ma, H, Zhang, H, Stoleriu, MG, Nowak, J, Nakayama, M, Bueno, M, Brands, J, Mora, AL, Lee, JS, Krauss-Etschmann, S, Dmitrieva, A, Frankenberger, M, Hofer, TP, Noessner, E, Moosmann, A, Behr, J, Milger, K, Deeg, CA, Staab-Weijnitz, CA, Hauck, SM, Adler, H, Goldmann, T, Gaede, KI, Behrends, J, Kammerl, IE & Meiners, S 2023, 'Antiviral CD8+ T cell immune responses are impaired by cigarette smoke and in COPD', The European respiratory journal, Jg. 62, Nr. 2, 2201374. https://doi.org/10.1183/13993003.01374-2022

GEN-COVID Multicenter Study Group 2023, 'CYP19A1 mediates severe SARS-CoV-2 disease outcome in males', Cell reports. Medicine, S. 101152. https://doi.org/10.1016/j.xcrm.2023.101152 

 

2022

Shan, L, Zhang, L, Zhu, X, Wang, Z, Fang, S, Lin, J, Wang, J, Li, N, Liu, H, Zhang, X, Feng, Y, Liu, J, Pan, J, Ye, G, Yu, X, Tufman, A, Katalinic, A, Goldmann, T, Petersen, F, Jiang, J, Geng, G & Yu, X 2022, 'Chinese never smokers with adenocarcinoma of the lung are younger and have fewer lymph node metastases than smokers', RESPIRATORY RESEARCH, Jg. 23, Nr. 1, S. 293. https://doi.org/10.1186/s12931-022-02199-z

Kasper, B, Yue, X, Goldmann, T, Gülsen, A, Kugler, C, Yu, X & Petersen, F 2022, 'Air exposure and cell differentiation are essential for investigation of SARS-CoV-2 entry genes in human primary airway epithelial cells in vitro', Frontiers in Medicine, Jg. 9, S. 897695. https://doi.org/10.3389/fmed.2022.897695

DZIF-TB cohort study group 2022, 'Gene expression signatures identify biologically and clinically distinct tuberculosis endotypes', The European respiratory journal, Jg. 60, Nr. 3. https://doi.org/10.1183/13993003.02263-2021

Kupsch, S, Eggers, LF, Spengler, D, Gisch, N, Goldmann, T, Fehrenbach, H, Stichtenoth, G, Krause, MF, Schwudke, D & Schromm, AB 2022, 'Characterization of phospholipid-modified lung surfactant in vitro and in a neonatal ARDS model reveals anti-inflammatory potential and surfactant lipidome signatures', European journal of pharmaceutical sciences, Jg. 175, S. 106216. https://doi.org/10.1016/j.ejps.2022.106216

Budczies, J, Kirchner, M, Kluck, K, Kazdal, D, Glade, J, Allgäuer, M, Kriegsmann, M, Heußel, C-P, Herth, FJ, Winter, H, Meister, M, Muley, T, Goldmann, T, Fröhling, S, Wermke, M, Waller, CF, Tufman, A, Reck, M, Peters, S, Schirmacher, P, Thomas, M, Christopoulos, P & Stenzinger, A 2022, 'Deciphering the immunosuppressive tumor microenvironment in ALK- and EGFR-positive lung adenocarcinoma', CANCER IMMUNOLOGY IMMUNOTHERAPY, Jg. 71, Nr. 2, S. 251-265. https://doi.org/10.1007/s00262-021-02981-w

Uchino, J, Goldmann, T & Kimura, H 2022, 'Editorial: Treatment for Non-Small Cell Lung Cancer in Distinct Patient Populations', Frontiers in Oncology, S. 838570. https://doi.org/10.3389/fonc.2022.838570

 

Head

Prof. Dr. rer. nat. Torsten Goldmann
Prof. Dr. rer. nat. Torsten Goldmann
+49 4537 / 188-2310
+49 4537 / 188-2290
This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Scientific staff

Dr. rer. nat. Sebastian Marwitz
Dr. rer. nat. Sebastian Marwitz
Stellvertretung / Deputy
+49 4537 / 188-6273
+49 4537 / 188-2290
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Dr. Dörte Nitschkowski
Dr. Dörte Nitschkowski
+49 4537 / 188-6273
+49 4537 / 188-2290
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Technical staff

Bettina Baron-Lühr
Bettina Baron-Lühr
+49 4537 / 188-4210
+49 4537 / 188-2290
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Ulrike Büchsler
Ulrike Büchsler
+49 4537 / 188-2720
+49 4537 / 188-2290
Angela Kelp
Angela Kelp
+49 4537 / 188-2190
+49 4537 / 188-2290
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Christian Rosero
Christian Rosero
+49 4537 / 188-6675
+49 4537 / 188-2290
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Vivien Sparr
+49 4537 / 188-4210
+49 4537 / 188-2290
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Jasmin Tiebach
Jasmin Tiebach
Leitende MTA / Senior MTA
+49 4537 / 188-6670
+49 4537 / 188-2290
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Last Update: 09.01.2024