Programmbereich Asthma und Allergie

Biofunktionelle Metabolite und Strukturen

Mission    Projekte    Drittmittel    Methoden    Publikationen    Mitarbeiter/innen

Methoden

The group of Biofunctional Metabolites and Structures possess a strong expertise in the isolation, purification protocols of different environmental samples, along with the chemical and structural characterization of the obtained compounds. Moreover, we have experience in immunological test of technically challenging lipid molecules.

  • Various extraction/purification protocols aiming for isolation and purification of components of microbial and environmental origin (dust, house dust mites, pollen).
  • Various chemical analyses, aiming for the compositional analyses of isolated carbohydrates, glycolipids, lipids
  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
  • Western blot
  • High Performance-Thin Layer Chromatography (TLC, HPTLC)
  • Ultracentrifugation
  • Gel permeation chromatography
  • Hydrophobic-interaction chromatography
  • Affinity chromatography
  • Anion exchange chromatography
  • Gas chromatography (GC)
  • Gas chromatography-Mass spectrometry (GC-MS)
  • High-performance liquid chromatography (HPLC)
  • High-performance anion-exchange chromatography (HPAEC)
  • NMR spectroscopy
  • Mass spectrometry
  • Flow cytometry
  • Cell culture
  • ELISA
  • RT-PCR
  • Human and mouse immune cell isolation