Programmbereich Asthma und Allergie

Experimentelle Pneumologie

Mission    Projekte    Drittmittel    Methoden    Publikationen    Mitarbeiter/innen

Techniques

In-vitro Culture of Primary Cells and Tissues

  • air-liquid-interface culture of primary human airway epithelial cells
  • microdissection and explant culture of murine proximal and distal airways
  • isolation and stimulation of primary murine mast cells
  • isolation and co-culture of human peripheral blood immune cells (special expertise: basophils).
  • production and purification of monoclonal antibodies


Analysis of Lung Structure

  • design-based stereology
  • fluorescence and immuno histochemistry


Analysis of Lung Cell and Molecular Biology

  • enzyme-linked immune sorbent assay (ELISA)
  • cytometric bead array s(CBA)
  • magnetic cell sorting
  • flow cytometry
  • Western blotting
  • RNA-isolation, cDNA synthesis, quantitative real-time RT-PCR
  • analysis of mast cell activation and degranulation by flow cytometry


Protein Biochemistry

  • protein purification and chromatographic techniques
  • recombinant protein expression (E. coli, insect cells, HEK cells)